DNA

Part:BBa_K4747002:Design

Designed by: Liu Lantian   Group: iGEM23_SUSTech-MED   (2023-10-11)


xisF4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10


Design Notes

To construct vectors for aiiA/ytnP/wspF/yhjH expression


Source

xisF4 is an excisionase gene found in Pf4 filamentous prophage, playing a crucial role in controlling the lysis-lysogeny decision by activating Pf4 phage replication via upregulating the phage initiator gene (PA0727)​​

References